I Never Dreamed I'd Grow Up to Be a Super Cool Urologist But Here I am Killin' It
by Mary Lou Darling
This title provides a contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer q...
Culture of Animal Cells
In this edition, the author provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of animal cells. Containing step-by-step laboratory procedures, detailed instructions for reagent preparation, and information on latest specialized equipment and supplies, this laboratory manual includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.
Fundamental Laboratory Approaches for Biochemistry and Biotechnology
by Alexander J. Ninfa and David P. Ballou
Written by active scientists, this book provides readers with a logical framework for conducting modern biochemical and molecular biology research. The authors write with passion, and their love of biochemical research shines through on every page.
Nonhuman Primates and Medical Research
Bioluminescence (Methods in Molecular Biology, v. 102)
A state-of-the-art collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors - all hands-on researchers who have perfected their protocols to a high degree of reproducibility - illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wid...
Molecular Diagnostics
Basics of Quality Assurance for Intermediate and Peripheral Laboratories (WHO Regional Publications)
by M.M. El-Nageh
Obsessive Compulsive Disorders
Obsessive Compulsive Disorder is now recognised as one of the most common psychiatric disorders, and the 10th most disabling of all medical disorders. Major advances in our understanding of its neurobiological basis and the discovery of robust treatments offer new hope for sufferers of the condition. Yet, sadly, people with OCD continue to remain under-diagnosed and inappropriately treated. Bringing together an international team of experts in one concise volume, Obsessive Compulsive Disorders...
Short Protocols in Cell Biology
Providing condensed descriptions of more than 500 methods compiled from Current Protocols in Cell Biology, this text thoroughly explores cell biology in an easily accessible, hands-on format. Short Protocols in Cell Biology is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of cell biology methods. Key Features: Designed to provide quick access to step-by-step instructions for the essential methods used in every maj...
This book provides a detailed view of the molecular structures of DNA and RNA and how they are recognised by small molecules and proteins. Extensive source material is provided, including information on relevant web sites and computer programmes. The major methods of structural investigation for nucleic acids: X-ray crystallography, NMR, and molecular modelling are reviewed and their scope and limitations (in the context of nucleic acids) discussed. Also covered are the conformational features...
Zebrafish (Practical Approach, #261)
The zebrafish has become one of the most important model organisms to study biological processes in vivo. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. The next few years will see the completion of large scale initiatives that exploit the zebrafish as a model system for the understanding of gene function in vertebrates, including the sequenci...
Protein-Ligand Interactions: Structure and Spectroscopy (Practical Approach, #243)
The two Practical Approach volumes on protein-ligand interaction do not comprise a comprehensive compilation of all the methods that can be used to investigate protein-ligand interactions. Instead, they are a selection of the most useful and easily applied methods and will be an invaluable guide to the principal techniques used to study the interactions of proteins and ligands. This second volume covers the major spectroscopic methods: FTIR, Raman, and fluorescence spectroscopy; circular dichroi...
Eukaryotic mRNA Processing (Frontiers in Molecular Biology, #17)
This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example during embryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the st...
Data for Biochemical Research
This book is designed to supply research workers in biochemistry and related fields with precise factual information about the compounds, reagents, and techniques most frequently used in the laboratory. The opportunity has been taken in this third edition to improve the presentation of the data, delete many obscure compounds, and add new material of current importance in biochemistry and molecular biology. Functional grouping of compounds has been emphasized so that users can see what is availab...
C. elegans (Practical Approach, #213)
Caenorhabditis Elegans has been a popular model organism for biological research for over thirty years and has been used to investigate many aspects of animal development, for example apoptosis, the Hox genes, signal transduction pathways, and the development of the nervous system. It has recently taken on new importance with the publication of the entire genome sequence in 1998. The first chapter gives all the basic information on C. elegans required to use it: it's natural history, anatomy, li...
Proteolytic Enzymes (Practical Approach, #247)
Like the popular first edition, this new edition of Proteolytic Enzymes emphasizes practical aspects of the handling, characterization, inhibition, and use of proteolytic enzymes giving general advice and specific examples. The text and protocols have been thoroughly updated to take account of the advances made in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes e.g. apoptosis and new technological developments e.g. in recombinan...
Gentechnisches Labor – Leitfaden für Wissenschaftler
by Kirsten Bender and Petra Kauch
Dieses Buch liefert Ihnen einen umfassenden und praxisnahen Überblick zu Fragen zum Gentechnikgesetz und seiner Rechtsverordnungen im Laboralltag. Ob Transformation bei Bakterien oder virale Gentransfermethoden bei höheren Zellen, die Kenntnis der rechtlichen Rahmenbedingungen und der Sicherheit in den Labors spielt bei allen angewandten Methoden die wichtigste Voraussetzung. Diese werden in Deutschland im Wesentlichen durch das Gentechnikgesetz und die Gentechnik-Sicherheitsverordnung gewährl...