This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example during embryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and
catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and protein factors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the
mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processes and to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process of insertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading
researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.
- ISBN10 0199634173
- ISBN13 9780199634170
- Publish Date 7 August 1997 (first published 1 August 1997)
- Publish Status Out of Print
- Out of Print 16 March 2018
- Publish Country GB
- Imprint Oxford University Press
- Format Paperback
- Pages 396
- Language English