Somatic cell genetics is an exciting and rapidly expanding field of research. Since descriptions of the major experimental techniques in the field are scattered throughout various journals and other publications, there is a real need for a single reference source for both established investigators and students in the field. In addition, technical reports are frequently abridged such that many researchers are discouraged from attempting to adopt the appropriate methodology. This book, therefore, describes in detail the many recent technical advances in such areas of somatic cell genetics as transfer mediated by liposomes, erythrocyte ghosts, chromosomes, micro cells, mito- chondria, and isolated nuclear DNA. These techniques have increased our understanding of the organization and regulation of eukaryotic cells. The production of antibiotic-resistant cell lines and their use in studying cytoplasmic inheritance are also included. Evidence for the cytoplasmic regulation of nuclear gene expression in eukaryotic cells is rapidly accumu- lating following the characterization of cytoplasmic mutations.
The produc- tion of nuclear-coded mutations, their use in standard cell hybridization, and recent advances in techniques for fusing whole cells or cell components are also described.
- ISBN10 0306410400
- ISBN13 9780306410406
- Publish Date 30 September 1982
- Publish Status Active
- Publish Country NL
- Publisher Kluwer Academic Publishers Group
- Imprint Kluwer Academic / Plenum Publishers
- Edition Abridged edition
- Format Hardcover
- Pages 568
- Language English