The aim of "Single Cell Diagnostics: Methods and Protocols" is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR). As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.
- ISBN10 159745298X
- ISBN13 9781597452984
- Publish Date 1 December 2007 (first published 15 June 2007)
- Publish Status Active
- Publish Country US
- Imprint Humana Press Inc.
- Format eBook
- Language English