Series:

Current Topics in Microbiology and Immunology

2 primary works

Book 67

Cover of Current Topics in Microbiology and Immunology / Ergebnisse Der Microbiologie Und Immunitatsforschung
Prominent progress in molecular biology was only made when it became possible to separate functionally distinct molecules by taking advantage of their biophysical properties. Likewise, the analysis of the functions of hetero- geneous populations of immunocompetent cells, as to the functional properties of their various subpopulations, can not be done until these can be isolated in reasonably pure form by selective fractionation. During the last few years significant advances have been made in this field, and cells have been separated according to size, density or charge (MILLER et aI. , 1969; SHORTMAN, 1968; ANDERSSON, 1973 c), or by taking advantage of more specific surface markers to allow selective depletion or enrichment of a given subpopulation of cells (WIGZELL and ANDERSSON, 1971). Although separation techniques have been used in a variety of cellular systems, they have been particularly useful in the study of reticuloendothelial cells and primarily in the study of cells partici- pating in the immune responses.
Quite extensive reviews have been written which well cover the methods used for separation of cells and the results obtained with the various approaches (WIGZELL and ANDERSSON, 1971; SHORTMAN, 1972). To review this work is becoming a more and more voluminous task. As data rapidly accumulate, we will not try to make such a complete review.

Book 320

Cover of RNA Interference

RNA Interference

by Patrick J Paddison and P. K Vogt

Published 17 December 2007

In the last few years the major effect that RNAi has had in invertebrate systems is beginning to take hold in mammalian systems through both single gene knockdown experiments and genome-scale screens. In the next decade, there will no doubt be both notable successes and failures as we attempt to apply this genetic tool to various biological problems. Through the introduction of RNAi, mammalian systems have finally gained admittance to the pantheon of model genetic systems.