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In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, st...

Discusses New Advancements to Improve Existing Simulations of Plant NitrogenWritten by research pioneers and leading scientists in the area of agricultural systems, Quantifying and Understanding Plant Nitrogen Uptake for Systems Modeling comprehensively covers plant N uptake in agricultural system models, especially for building soil-plant system m

he biological sciences are dominated by the idea that cells are the functionally autonomous, physically separated, discrete units of life. TThis concept was propounded in the 19th century by discoveries of the cellular structuring of both plants and animals. Moreover, the ap parent autonomy of unicellular eukaryotes, as well as the cellular basis of the mammalian brain (an organ whose anatomy for a long while defied attempts to validate the idea of the cellular nature of its neurons), seemed to...

This volume describes and integrates the techniques and fundamentals of more than a decade of revolutionary advances in both chromatographic and mass spectrometric technologies that have enabled the direct investigation of biomacromolecules per se and have provided the analytical power base to usher in the new fields of proteomics and systems biology. It also covers new biophysical applications such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand inter...

Based on the proceedings of a Symposium held during the 2002 World Congress of the Oxygen Club of California, 2002.

An overview of speciation theory reveals an increasingly held view that many events leading to the origin of new species occur in transient, unstable populations. A transient, unstable population should be under stood as a fast episodic phase in a population subjected to genetic and environmental factors that tend to disrupt its cohesive, balanced genome architecure, thus enhancing its probability to produce a new species. Striking the core of Darwinian thought, some authors claim that these. pr...

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. This volume...

This long-awaited second edition covers the major changes that have occurred in the field over the last decade  Completely revised with the most up-to-date research and including brand new chapters, Annual Plant Reviews, Volume 50: Plant Mitochondria, 2nd Edition presents the multifaceted roles of mitochondria in plants. The book starts with a short history of plant mitochondrial research; discusses how coevolution shaped plant mitochondrial gene expression; explains control of number, shape, si...

This book consists of a series of reviews on selected topics within the rapidly and vastly expanding field of membrane biology. Its aim is to highlight the most significant and important advances that have been made in recent years in understanding the structure, dynamics, and functions of cell membranes. The progress that is being made in research in this field is due to the application of integrated experimental ap- proaches, utilizing sophisticated and novel techniques in molecular biology, c...

This book is based on two keywords: Bioradical and ESR. Bioradical is a newly coined word which encompasses paramagnetic species in biological systems, such as active oxygen radicals and transition metal ions. Research on the structure and function of bioradicals has been attrac- ting growing attention in the field of biological science, and comprehensive investigations from many fields are helping to understand the real features of these species. ESR spectroscopy also has interdisciplinary feat...

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).