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Das Buch enthalt folgende Beitrage: C. Harbour, J.P. Barford, K.-S. Low, Sydney, Australia: Prozessentwicklung fur Hybridoma-ZellenA.J. MacLeod, Edinburgh, UK: Die Verwendung von Plasmaproteinfraktionen als Zusatz zum Medium von TierzellkulturenM. Morandi, A. Valeri, Siena, Italy: ss-Interferon Produktion im industriellen MassstabJ. Engels, E. Uhlmann, Frankfurt, FRG: GensyntheseH. Schwab, Graz, Austria: Rekombinante DNA-Techniken zur Verbesserung industriell genutzter Mikroorganismenstamme

First Published in 2000. Routledge is an imprint of Taylor & Francis, an informa company.

This contributed volume reviews the recent progress in our understanding of membrane transport in yeast including both Saccharomyces cerevisiae and non-conventional yeasts. The articles provide a summary of the key transport processes and put these in a systems biology context of cellular regulation, signal reception and homeostasis.  After a general introduction, readers will find review articles covering the mechanisms and regulation of transport for various substrates ranging from diverse n...

This book presents a comprehensive discussion on the heterogeneity existing between different types of stem cells within the same tissue. As the functions of these stem cells vary, this is very important for the application of stem cells in cell therapy. This book describes the many recent developments that have revealed completely different roles of distinct stem cells within the same organ.Stem Cells Heterogeneity in Different Organs provides a timely update on the current information on stem...

Biochemistry and molecular biology are among the most rapidly emerging areas in the life sciences. Indeed, a number of important advances have been made with fungi and yeasts since the first edition of this volume was published in 1996. Still further, the influence that genomics projects have had on the design and interpretation of experiments in almost all areas is truly impressive. The availability of large amounts of sequence data has quickly altered the scope and dimensions of genetics and b...

Aluminum, bound almost exclusively to oxygen in various combinations, is the most abundant metal in the earth's crust and, therefore, of great commercial potential. Once methods were developed (in the 1880's) to free useable quantities of the element from oxygen, applications for the element began developing rapidly. This growth has resulted in the ubiquity of the metal in today's world. Therefore it can be found intentionally introduced in many products in direct contact with human beings. It i...

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which h...

This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and...

This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, an...

This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducibl...

This volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials...

The actin cytoskeleton plays a central role in many cellular processes including cell motility, cytokinesis, endocytosis and phagocytosis. The structure and dynamics of the actin cytoskeleton is regulated by a large number of proteins that interact with monomeric and/or filamentous actin. Actin monomer binding proteins provides a comprehensive view on actin monomer-binding proteins and the mechanisms by which they contribute to actin dynamics and various actin-dependent cellular processes. This...

Viruses: Biology, Application, and Control is a concise textbook for advanced undergraduate and graduate students covering the essential aspects of virology included in biomedical science courses. It is an updated and expanded version of David Harper’s Molecular Virology, Second Edition. Focusing on key mechanisms and developments, Viruses presents many new recent scientific advances, including virus evolution, emerging infections, virus extinction, control of infections, antiviral drugs, gene t...

This book summarizes the latest studies on plant reproduction and multiple aspects of signaling in reproductive development. It also presents the most advanced processes in CrRLK1L receptor and RALF peptide studies during plant development. Focusing on signaling in pollen tube integrity and sperm release regulation, it provides significant insights into the BUPS-ANX receptor complex and the corresponding ligands RALF4/19 to promote pollen tube growth with proper cell integrity. It also proposes...

Sequencing projects have revealed the presence of at least several hundred receptor kinases in a typical plant genome.  Receptor kinases are therefore the largest family of primary signal transducers in plants, and their abundance suggests an immense signaling network that we have only just begun to uncover.  Recent research findings indicate that individual receptor kinases fulfill important roles in growth and development, in the recognition of pathogens and symbionts or, in a few examples, in...

Of all the zoological classes the insects are the most numerous in species and the most varied in structure. Estimates of the number 18 of species vary from 1 to 10 million, and 10 individuals are es­ timated to be alive at any given moment. In their evolution, in­ sects are relatively ancient and, therefore, they have proved to be a phenomenally successful biological design which has survived unchanged in its basic winged form during the last 300 m. y. In­ sects were the first small animals to...

This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Met...

The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins.  In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function,...

This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Met...

This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and o...

This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their res...

This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materia...