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This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical meth...

As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with...

Covering 100 years of zoological research, the Handbook of Zoology represents a vast store of knowledge. Handbook of Zoology provides an in-depth treatment of the entire animal kingdom covering both invertebrates and vertebrates. It publishes comprehensive overviews on animal systematics and morphology and covers extensively further aspects like physiology, behavior, ecology and applied zoological research. Although our knowledge regarding many taxonomic groups has grown enormously over the la...

The mouse is a perfect model organism to study mammalian, and thus indirectly also human, embryology. Most scientific achievements that have had an important impact on the understanding of basic mechanisms governing embryo development in humans, originated from mouse embryology. Stem cell research, which now offers the promise of regenerative medicine, began with the isolation and culture of mouse embryonic stem cells by Martin Evans (who received the Nobel Prize in medicine in 2007 for this ach...

Experts in the field of cellular and molecular biology of myelination present their latest results. Various techniques were used such as tissue culture, transplantation, transfection, immunocytochemistry and approaches to study the phylogenetic aspects of myelination as well as molec- ular biology. Concerning glial cells: hormones and second messengers play an important role in the regulation of the phenotype and the expression of myelin components at the transcriptional level; these findings ar...

Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body’s natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so...

Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells, by S. Jakobs, S. Stoldt, and D. Neumann*  Advanced Microscopy of Microbial Cells, by J. A. J. Haagensen, B. Regenberg, and C. Sternberg * Algebraic and Geometric Understanding of Cells, Epigenetic Inheritance of Phenotypes Between Generations, by K. Yasuda *  Measuring the Mechanical Properties of Single Microbial Cells, by C. R. Thomas, J. D. Stenson, and Z. Zhang*  Single Cell Analytics...

This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagen...

Leading experts critically summarize the state of knowledge concerning the molecular, anatomical, physiological, and behavioral aspects of NPY and its congeners. Each article provides a comprehensive and in-depth survey, an overview of the role of NPY in the discipline covered, a discussion of the likely future direction that the field will take, and an up-to-date bibliography. Chapters include a treatment of the evolution of the PP family of genes, the structure of the NPY gene, and the distr...

In this book, the major paradigm-shifting discoveries made in the past century on key cellular nanomachines are described in great detail: their complex yet precise and elegant design and function, as well as the diseases linked to their dysfunction and the therapeutic approaches to overcome them. The major focus of this book is the “porosome” nanomachine, the universal secretory portal in cells. This is an ideal book for students, researchers, and professionals in the fields of nanoscience and...

This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topi...

The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I: concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the constru...

Photosynthesis and the biochemistry of the chloroplast are dealt with in this textbook, which is now available in an updated and corrected paperback edition. It places great emphasis on developments in our knowledge of the Calvin cycle and its regulation, C 4 and CAM plants, nitrogen and sulphur metabolism, phenolic compounds, photorespiration, and the toxic effects of oxygen on plant tissues.

Cell death has many different meanings to scientists in different disciplines, who use very different approaches in its study. There is no single definition of cell death which can be accepted and used by all biologists. Yet it is becoming increasingly clear that understanding how and why cells die is crucial to understanding many biological and pathological processes. Cells may die because they are damaged, infected or old. What triggers destruction rather than repair? Do the effects of insult...

While the theoretical basis of centrifugal separation remains the same, recent years have seen dramatic changes in both the design of centrifuges and the range of people that use them. This study is designed to reflect these changes by providing detailed protocols covering all types of separation, from DNA to cells. Guidelines are also given to help researchers devise new types of separation protocols. The book includes program source codes for calculation and simulation programs that are benefi...

A series of authoritative reviews of current progress in plant molecular and cellular biology. Each volume contains papers on topics in areas of molecular genetics, plant micro-organism interactions, and cell biology. The reviews are concentrated on areas of rapid development, particularly where modern techniques are allowing new insights to be gained. They are published rapidly after completion due to the pace of research in this area.

Volume 2 covers cell renewal and organization in the gastrointestinal epithelia, and the conditional renewal populations.

The sixth in a series of authoritative reviews of current progress in plant molecular and cell biology. Each volume contains papers on topics in areas of molecular genetics, biochemical and cell genetics, plant micro-organism interactions, and cell biology. The reviews are concentrated on areas of rapid development particularly where modern techniques are allowing new insights to be gained. In order to cope with the pace of research in this active area, the reviews are published within four to s...

Glial Neuronal Signaling fills a need for a monograph/textbook to be used in advanced courses or graduate seminars aimed at exploring glial - neuronal interactions. Even experts in the field will find useful the authoritative summaries of evidence on ion channels and transporters in glia, genes involved in signaling during development, metabolic cross talk and cooperation between astrocytes and neurons, to mention only a few of the timely summaries of a wide range of glial - neuronal inte...

The Golgi apparatus (GA) is typically comprised of a series of five to eight cup-shaped, membrane-covered sacs called cisternae that look something like a stack of deflated balloons. The GA is often considered the "distribution and shipping department" for the cell's chemical products. This book traces the first 100 years of GA discovery from the first published accounts from Pavia, Italy, in 1898 to the Centenary Celebration in Pavia, Italy, in 1998 to our most recent discoveries. It summarizes...