Structure and Function of the Genetic Apparatus (NATO Science Series A, #98)
The Fourth Course of the International School of Pure and Applied Biostructure, a NATO Advanced Study Institute, was held September 18-31, 1983 at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. The subject of the Fourth Course, which was co-sponsored by national and international agencies, was "Structure and Function of the Genetic "Appara- tus. " Participants from 15 countries around the world attended the course. The study of the genetic apparatus is one of humanity's most...
Genetics and Epigenetics of Genitourinary Diseases
Genetics and Epigenetics of Genitourinary Diseases aims to provide a comprehensive collection of data on genetics and epigenetics in almost all genitourinary diseases for researchers, clinicians and graduate students. It discusses several genetic and epigenetic changes like DNA methylation, mutations, Single Nucleotide Polymorphisms (SNPs), DNA rearrangements, chromosomal remodeling, MSI (Microsatellite Instability) and Chromosomal Instability (CIN) in genitourinary cancers. It is split into 15...
Adenovirus Methods and Protocols (Methods in Molecular Biology, v. 21) (Methods in Molecular Medicine, #130)
A cutting-edge collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems a...
Pharmaceutical Stability Testing
Stability testing is a critical piece of a drug development program that assesses a potential drug's shelf life and required storage conditions. Pharmaceutical Stability Testing: A Practical Guide provides a comprehensive guide to the approaches and regulations covering stability testing. The book helps pharmaceutical personnel organize and conduct drug stability tests by describing the many different aspects of drug stability programs, the different types of study that are required, and the app...
Dr. Jacqueline N. Crawley, author of the First and Second Editions of What’s Wrong with My Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice,continues to field calls and e-mails from molecular geneticists who ask: how do I run behavioral assays to find out what’s wrong with my mouse? Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavio...
Signal Transducers and Activators of Transcription (STATs)
The year 2003 marks the tenth anniversary of the first use of the acronym "Stat" (also written "STAT") in the scientific literature for a family of transcription factors which rapidly transduce cytokine-and growth factor elicited signals from the plasma membrane to the cell nucleus thereby activating gene transcription (thus, . s. ignal Transducers and Activators of Transcription). From those beginnings, the field of STAT transcription factors, their related regulatory molecules and their biolo...
Human genetics is not the playground of science alone. Genetics concerns all of us, for we all have DNA, genes, genomes, and chromosomes. In fact, our genes determine our appearance and our behaviour. They even define our talents and our health risks. The authors of The Human Recipe explain clearly and with humour what exactly is understood by human genetics. With anecdotes and topical examples they demonstrate how genetics interferes with our everyday lives. What if a DNA analysis reveals that...
Manipulating the Mouse Embryo
by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard R. Behringer
Updated 2024 to include significant additional material from 2018 to 2024. At the 16 week foetal stage, Paul Whymark’s brain development was frozen as a consequence of the drug, Thalidomide. His journey is one of considerable self discovery, but also reveals greater societal insights. “16 Weeks and Everything After...” uncovers the remarkable fact that over the last 50-60 years the establishment has failed to genuinely independently review Thalidomide, and as a consequence, harms on developing l...
On the last day of August in the year 2020 two girls are born. Genetic science enables the long life of one of them to be happy and free of suffering; she finally dies in 2140. In stark contrast, genes fill the life of the other with discrimination and oppression, and after enduring much misery she dies young, in 2048. These 'future histories' of two individuals provide a striking opening to Genetic Destinies. In the chapters that follow, Peter Little discusses the reality of the power of gene s...
This book is a state-of-the-art catalogue of viral and cis-acting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. It describes in detail the essential techniques for propagating cells and cell lines, DNA transfer, drug selection and gene amplification, expression cloning, subtractive hybridizaton, retro-virus mediated gene transfer.
Mutation Detection (Practical Approach, #188)
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
Antisense Technologies (Practical Approach, #185)
Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic a...
In February 2001, two separate teams published the first working drafts of the entire human genome, marking an achievement that is certainly one of the seminal developments in our understanding of human biology. A grasp of the function of each gene will radically change how we diagnose and prevent diseases and adminster treatments. But the drive to turn the completed sequence into practical knowledge is fraught with complexity. This volume, a summary of the eponymous symposium, gives the student...
Cardiovascular and Musculoskeletal Systems (Monographs on Pathology of Laboratory Animals, No 9)
This is the ninth volume in a series dealing with induced lesions in laboratory animals. The information on pathology and toxicology documented in the series is an aid to scientific institutions, industry and government agencies charged with the safety testing of food, drugs and chemicals.
Mobile Genetic Elements (Methods in Molecular Biology, #260)
by Wolfgang J. Miller and Pierre Capy
Leading experts describe in step-by-step detail their most productive transposon-based methods and strategies for studying genome structure, function, and evolution. These readily reproducible techniques cover a wide range, including mutagenesis, transgenesis, gene silencing, and molecular systematics. Among the highlights are a series of DNA hybridization methods for analyzing the distribution and dynamics of mobile DNA at the hosts' genomic level, techniques for studying LTR retrotransposons i...
Cloning Systems (Genome Analysis Laboratory Manual S., v. 3)
Bioreactor Systems for Tissue Engineering (Advances in Biochemical Engineering/Biotechnology, #112)
The editors of this special volume would first like to thank all authors for their excellent contributions. We would also like to thank Prof. Dr. Thomas Scheper, Dr. Marion Hertel and Ulrike Kreusel for providing the opportunity to compose this volume and Springer for organizational and technical support. Tissue engineering represents one of the major emerging fields in modern b- technology; it combines different subjects ranging from biological and material sciences to engineering and clinical...
Biochemistry And Genetics of RecQ-Helicases provides a background into the role of helicases in general and RecQ helicases specifically in DNA repair. Helicases- enzymes which break down hydrogen bonds between nucleic acid strands in a nucleoside triphosphate-dependent manner-are ubiquitous in biology, participating in processes as diverse as replication, repair, recombination, transcription, and translation. The RecQ-family helicases are a group of helicases which have important roles in...