Human genetics is not the playground of science alone. Genetics concerns all of us, for we all have DNA, genes, genomes, and chromosomes. In fact, our genes determine our appearance and our behaviour. They even define our talents and our health risks. The authors of The Human Recipe explain clearly and with humour what exactly is understood by human genetics. With anecdotes and topical examples they demonstrate how genetics interferes with our everyday lives. What if a DNA analysis reveals that...
Comparative Genomics (Computational Biology, #1)
A comprehensive account of genomic rearrangement, focusing on the mechanisms of inversion, translocation, gene and genome duplication and gene transfer and on the patterns that result from them in comparative maps. Includes analyses of genomic sequences in organelles, prokaryotes and eukaryotes as well as comparative maps of the nuclear genomes in higher plants and animals. The book showcases a variety of algorithmic and statistical approaches to rearrangement and map data.
Structural Biology and Functional Genomics (Nato Science Partnership Subseries: 3, #71)
Biomedical research will be revolutionised by the current efforts to sequence the human genome and the genomes of model organisms. Of the newly sequenced genes, 50% code for proteins of unknown functions, while as little as 5% of sequences in mammalian genomes code for proteins. New, genome-wide approaches are needed to draw together the knowledge that is emerging simultaneously in a number of fields of genome research. This volume is a high-level survey of the newly emerging concepts o...
On the last day of August in the year 2020 two girls are born. Genetic science enables the long life of one of them to be happy and free of suffering; she finally dies in 2140. In stark contrast, genes fill the life of the other with discrimination and oppression, and after enduring much misery she dies young, in 2048. These 'future histories' of two individuals provide a striking opening to Genetic Destinies. In the chapters that follow, Peter Little discusses the reality of the power of gene s...
Mutation Detection (Practical Approach, #188)
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
Gene Quantification (Advanced Biomedical Technologies)
Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate...
The main theme of this book is how reproduction in fungi is controlled by genetic and environmental factors. The genetics of fungi is at a crossroads - the methods of classical genetics are giving way to those of recombinant DNA technology. Reproduction in Fungi takes stock of what has been learned to date and points the way to future research.
Genetics and Evolution of Aquatic Organisms
This volume brings together, for the first time, a wide range of up-to-the-minute and traditional techniques and approaches to the study of genetics of organisms living in freshwater or marine habitats. Carefully edited chapters are headed by broad review articles against which are set a number of more specific experience papers which demonstrate the breadth and range of approaches currently being undertaken.
DNA Barcodes (Methods in Molecular Biology, #858)
A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life inc...
Non Coding RNAs in Plants (RNA Technologies)
In the most recent years, each of the RNA silencing pathways of plants have appeared to generate ncRNAs with dedicated functions, specialized biological activities and specific functional scopes. RNA silencing plays a crucial role in coordinating the expression, stability, protection and inheritance of eukaryotic genomes. It compromises several mechanisms, that invariably depend on core small non coding RNAs and that achieve dedicated sequence-specific functions. RNA silencing has been recognize...
Heterologous Gene Expression in E.coli (Methods in Molecular Biology, #705)
Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modificatio...
Mouse Development (Results and Problems in Cell Differentiation, #55)
The mouse is a perfect model organism to study mammalian, and thus indirectly also human, embryology. Most scientific achievements that have had an important impact on the understanding of basic mechanisms governing embryo development in humans, originated from mouse embryology. Stem cell research, which now offers the promise of regenerative medicine, began with the isolation and culture of mouse embryonic stem cells by Martin Evans (who received the Nobel Prize in medicine in 2007 for this ach...
Cell Fusion (Methods in Molecular Biology, #475)
Exciting work in the past decade has revealed commonalities and differences among individual cell fusion events. In Cell Fusion: Overviews and Methods, a team of leading experts provide a collection of overviews that outline our current understanding of cell fusion and methods that present classic and state-of-the-art experimental approaches in a variety of systems. Divided into two convenient parts, the volume begins with nine overviews which describe different cell fusion events in models fro...
DNA-Protein Interactions (Methods in Molecular Biology, #543)
Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. Addressing topics such as chromatin immunopreci...
Plant Reverse Genetics (Methods in Molecular Biology, #678)
After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, invo...
Yeast Genetic Networks (Methods in Molecular Biology, #734)
Regulation of gene expression, a major determinant of gene activity, occupies a central place in molecular biology. Yeast Genetic Networks: Methods and Protocols covers approaches to the systems biological analysis of small-scale gene networks in yeast. Divided into four convenient sections, this detailed volume discusses the methods used to analyze gene expression quantitatively, presents a collection of mathematical and computational tools to analyze stochasticity, adaptation, sensitivity in s...
Permutiertes Symptomenregister Zur Diagnostik Genetisch Bedingter Syndrome Und Merkmalskombinationen
by Regine Otto Witkowski Prokop
Molecular Cytogenetics (Methods in Molecular Biology, #204)
The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagn...
Mammalian Artificial Chromosomes (Methods in Molecular Biology, #240)
In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion. On that occasion, a small group of investigators convened from many countries and presented early evidence of the possibility of assembling basic units of mammalian chromosomes into artificial constructs (or, indeed, red- ing the relevant components to more manageable dimensions and defined c- stitution). Progress in the following years has be...
Essential Molecular Biology: Volume I (Essential Molecular Biology, #234) (Practical Approach)
The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I: concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the constru...
Combining medical knowledge with sympathetic common sense, this completely updated comprehensive handbook offers help and advice to all parents and carers of children with Down's syndrome, and shows them how to help their children flourish and reach their full potential. Written in association with the Down's Syndrome Association, this book goes beyond the average introduction to Down's syndrome and addresses the questions, practical or otherwise, that parents will often find themselves asking,...
Johnson's book describes the effects of genetic mutations on the mammalian skeleton, and their usefulness as a tool to help us understand how the skeleton develops. Using the mouse as a primary source, but with mutations in other laboratory animals discussed where appropriate, the author considers the precartilaginous (mesenchymal), cartilaginous, and bony skeletons, regional anatomy (axial and appendicular skeletons, the skull, face, palate, and teeth), and the interactions between genes and te...
Antisense Technologies (Practical Approach, #185)
Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic a...
Signal Transducers and Activators of Transcription (STATs)
The year 2003 marks the tenth anniversary of the first use of the acronym "Stat" (also written "STAT") in the scientific literature for a family of transcription factors which rapidly transduce cytokine-and growth factor elicited signals from the plasma membrane to the cell nucleus thereby activating gene transcription (thus, . s. ignal Transducers and Activators of Transcription). From those beginnings, the field of STAT transcription factors, their related regulatory molecules and their biolo...