Molecular Toxicology Protocols (Methods in Molecular Biology, #1105)
Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of...
In Situ Hybridization Protocols (Methods in Molecular Biology, #2148)
In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Auth...
Structural Genomics (Methods in Molecular Biology, #2199)
The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, expe...
Promoter Associated RNA (Methods in Molecular Biology, #1543)
This volume is divided in four sections; covering genome wide approaches, techniques for characterize of paRNA structural features are described, selecting pa-RNA, and paRNA therapeutic potential. Chapters describe how siRNAsdirected against paRNAs can be applied in vivo to modulate transcription of important genes controlled by paRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary ma...
Transcription Factors (Practical Approach, #201)
Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the...
Phylogenomics (Methods in Molecular Biology, #422)
The past decade has seen the emergence of a new field of scientific inquiry at the intersection of phylogenetics and genomics: phylogenomics. From one perspective, phylogenomics is defined as the use of large genomic data sets to aid in difficult phylogeny problems. Alternatively, phylogenomics may be described as the use of phylogeny and comparative analysis to infer processes of genome evolution. Regardless of how one defines the field, the two app- cations are intertwined. This volume is a co...
Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Methods in Molecular Biology, #353)
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are als...
Affinity Chromatography (Methods in Molecular Biology, #421)
Forty years after the term “affinity chromatography” was introduced, this mode of chromatography remains a key tool in the armory of separation techniques that are available to separation and interaction scientists. Affinity chromat- raphy is favored because of its high selectivity, speed, and ease of use. The rapid and selective isolation of molecules using affinity chromatography has allowed a better understanding of biological processes, accelerated the identi- cation of target molecules, and...
Antiviral RNAi (Methods in Molecular Biology, #721)
Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and...
RNA and DNA Editing (Methods in Molecular Biology, #718)
The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniq...
PCR Primer Design (Methods in Molecular Biology, #1275)
This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding kn...
Mr. Chesterton's long essay on eugenics and other evils was written in 1922, just a few years after the close of the 'Great War.' This war was not yet known as World War I, and it could not then be imagined that a greater calamity could be possible. Chesterton ends with the acidic observation that if his readers don't believe how toxic materialistic philosophies are, "neither would they believe though one rose from the dead." Prophetic; Chesterton would die in 1936, a few short years before the...
Catalog of Teratogenic Agents
Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods (Principles and Practice)
Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements.
Linkage Disequilibrium and Association Mapping (Methods in Molecular Biology, #376)
As researchers continue to make enormous progress in mapping disease genes, exciting, novel, and complex analyses have emerged. In Linkage Disequilibrium and Association Mapping: Analysis and Applications, scientists from around the world, who are leaders in this field, contribute their vast experience and expertise to produce a comprehensive and fascinating text for researchers and clinicians alike. The volume comprises four general sections: the first presents an overview and historical basis...
Microbial Gene Essentiality: Protocols and Bioinformatics (Methods in Molecular Biology, #416)
Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality usi...
Since the Human Genome Project was completed in 2003, the price of genome sequencing has dropped at a staggering rate. It's as if the price of a Ferrari went from $350,000 to a mere $0.40. Through breakthroughs by Dr. Ashley's team at Stanford, along with other impressive groups around the world, analyzing the human genome has decreased from an almost impossible, multi-billion dollar cost to one test for under $1,000. For the first time, due to this affordable capability, we have within our...
For undergraduate courses in introductory-level Human Genetics, Biochemistry, and Molecular Biology courses. Also appropriate as a resource for law schools, legal clinics, and law enforcement offices. Part of the "Prentice Hall Exploring Biology Series", DNA Forensics explores the subject of modern DNA profiling in straightforward language, requiring and is aimed at students with little background in science or biotechnology. It raises controversial questions about the uses and potential misuse...
Classical Swine Fever and Related Viral Infections (Developments in Veterinary Virology, #5)
The history of research on hog cholera (HC)/classical swine fever (CSF) can be roughly divided into three phases which are characterized by the methods available at the time for demonstrati ng the causati ve agent. Phase covered the period before the viral etiology of HC was discovered by de Schweinitz and Dorset (1904)*. Thereafter (Phase II) the detection of HC virus (HCV) was accomplished by laborious, time-consuming and costly pig inoculation experiments. This explains the extensive search f...
Versuche über Pflanzenhybriden (Ostwalds Klassiker Der Exakten Wissenschaften)
by Gregor Mendel