Essential Molecular Biology: Volume I (Essential Molecular Biology, #234) (Practical Approach)
The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I: concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the constru...
Antisense Technologies (Practical Approach, #185)
Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic a...
Bacteriophages (Methods in Molecular Biology, #1898)
Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Char...
Protein Purification Applications (Practical Approach, #245) (Practical Approach S.)
A companion volume to "Protein Purification Methods: A Practical Approach", this volume concentrates on the purification of proteins for specific purposes, and provides clear descriptions immediately useful in the laboratory. Specific applications include large-scale techniques (for industrial and biotechnological use), high quality purification (for medical uses) and procedures of interest to biochemists, physiologists and others investigating the structure of proteins.
Protein Purification Techniques (Practical Approach, #244)
Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have r...
Functional Genomics (Practical Approach, #235)
With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides a comprehensive treatment of the range of methods available for gene and protein expression profiling in a variety of systems. These include large-scale methods, such as cDNA microarrays, serial analysis of gene e...
PCR Methods Manual
Optimum Experimental Designs (Oxford Statistical Science, #8)
by A. C. Atkinson and A. N. Donev
A well-designed experiment is an efficient method of learning about the world. Because experiments in the field and in the laboratory cannot avoid random error, statistical methods are essential for their efficient design and analysis. In this book, the fundamentals of optimum experimental design theory are presented. In the first part of the part of the book, the advantages of a statistical approach to the design of experiments are discussed, and the ideas of models, least squares fitting, and...
"First Published in 1999, Routledge is an imprint of Taylor & Francis, an informa company."
This comprehensively updated and revised edition of the bestselling PCR is designed as an introduction to the technique and provides authoritative coverage of a wide range of current applications. Topics covered include PCR instrumentation, reagents and consumables, amplifying the correct product, cloning, and the modification of PCR products.
Meselson, Stahl, and the Replication of DNA
by Frederic Lawrence Holmes
In 1957 two young scientists, Matthew Meselson and Frank Stahl, produced a landmark experiment confirming that DNA replicates as predicted by the double helix structure Watson and Crick had recently proposed. It also gained immediate renown as a "most beautiful" experiment whose beauty was tied to its simplicity. Yet the investigative path that led to the experiment was anything but simple, Frederic L. Holmes shows in this masterful account of Meselson and Stahl's quest. This book vividly recon...
The Juvenile Skeleton in Forensic Abuse Investigations
Juvenile homicide and fatal maltreatment remain serious and pervasive problems in the developed world and especially in the United States, where in 2005 some 1,500 children died from neglect and physical abuse. Alarming statistics such as this, as well as an upsurge in the media attention paid to all things forensic, underscore the pressing need for the utmost rigor in the scientific investigation of child abuse cases. This well timed volume is a response to the climate of public and press inter...
A practical guide for oil and gas rig personnel, this book offers help in coping with injuries and illness in these hazardous environments.
Viral Applications of Green Fluorescent Protein (Methods in Molecular Biology, #515)
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written...
Advances in Clinical Cytometry, an Issue of the Clinics in Laboratory Medicine, E-Book (Clinics: Internal Medicine, #43)
Nachweisvermoegen Von Analysenverfahren
by Gunter Ehrlich and Klaus Danzer
Die grundsatzlichen Moeglichkeiten und Grenzen der Bewertung des Nachweisvermoegens von Analysenverfahren und -ergebnissen werden dargestellt. Diesbezugliche Leistungskriterien haben Bedeutung fur die Auswahl und den Vergleich von Verfahren fur die Spurenanalyse inbezug auf praktische Aufgabenstellungen, die Interpretation von Analysenergebnissen sowie die Entwicklung, Optimierung und Validierung von Analysenverfahren. Die gegenwartige Vielzahl von teilweise widerspruchlichen Bezeichnungsarten u...
Mapping Genomes (Genome Analysis Laboratory Manual S., v. 4)
The Writing of History in the Middle Ages