This work describes the achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.
- ISBN10 3540420509
- ISBN13 9783540420507
- Publish Date August 2001
- Publish Status Active
- Out of Print 15 October 2014
- Publish Country DE
- Publisher Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
- Imprint Springer-Verlag Berlin and Heidelberg GmbH & Co. K
- Format Hardcover
- Pages 267
- Language English