A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
- ISBN10 1617372323
- ISBN13 9781617372322
- Publish Date 7 March 2011 (first published 16 July 2010)
- Publish Status Out of Print
- Out of Print 12 April 2021
- Publish Country US
- Imprint Humana Press Inc.
- Edition 2002 ed.
- Format Paperback (US Trade)
- Pages 396
- Language English