John G. Toffaletti received a BS from the University of Florida in Gainesville and followed this with training in clinical chemistry at the University of North Carolina at Chapel Hill, where he earned a PhD in Biochemistry, then completed a Postdoctoral Fellowship in Clinical Chemistry at Hartford Hospital. Since completing these programs, he has worked in the Clinical Laboratories at Duke University Medical Center since 1979, where he is now Professor of Pathology, Director of the Blood Gas Laboratory, the Clinical Pediatric Laboratory, and several Outpatient Laboratories. He is also the Chief of Clinical Chemistry at the Durham VA Medical Center.

He has written or presented numerous workshops, books, study guides, chapters, and seminars on the interpretation of blood gas, cooximetry, ionized calcium, magnesium, lactate (sepsis), kidney function tests (creatinine, cystatin C, GFR), and viscoelastic testing (ROTEM and TEG). His research interests include sample collection, pre-analytical errors, analysis, and clinical use of these tests.