Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP's developed.
This book is co-published with Water Research Australia.
- ISBN10 1780407521
- ISBN13 9781780407524
- Publish Date 15 June 2015
- Publish Status Active
- Publish Country GB
- Imprint IWA Publishing
- Format eBook
- Pages 36
- Language English