Quantitation of mRNA Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals)

by Thomas Kohler, Dirk Lassner, Anne-Katrin Rost, Barbara Thamm, Barbara Pustowoit, and Harald Remke

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In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.
  • ISBN10 3540591923
  • ISBN13 9783540591924
  • Publish Date 23 October 1995
  • Publish Status Active
  • Out of Print 13 November 2014
  • Publish Country DE
  • Publisher Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
  • Imprint Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • Edition 1995 ed.
  • Format Hardcover
  • Pages 186
  • Language English